Anticancer Activity of Postbiotic Mediators Derived from Lactobacillus Rhamnosus GG and Lactobacillus Reuteri on Acute Lymphoblastic Leukemia Cells

Background: Leukemia remains a global health challenge, requiring the exploration of alternative therapies with reduced side effects. Probiotics, particularly Lactobacillus species, have gained attention because their potential anticancer properties. This study investigated and cytotoxic effects postbiotic mediators (PMs) derived from rhamnosus GG (LGG) reuteri (LR) on acute lymphoblastic leukemia (ALL) cells peripheral blood mononuclear (PBMCs). Materials Methods: The PMs were prepared by culturing LGG LR strains isolating supernatant. MTT assay assessed cell viability ALL Jurkat PBMCs, apoptosis analysis was conducted using flow cytometry. Quantitative real-time PCR also performed to analyze BAX, BCL-2, BCLX, FAS, p27 gene expression levels. Results: results showed that significantly in (P<0.05) but not PBMCs (P>0.05). Apoptosis revealed an increase apoptotic after treatment. Nevertheless, no statistically significant difference between treated untreated groups levels Conclusion: Findings suggest specific possess properties against cells. research highlighted promise as cutting-edge less toxic adjuvant therapeutic strategy cancer